Born in Sun Valley, Idaho, Isabella Boylston began dancing at the age of three. While training at the Academy of Colorado Ballet, she won the gold medal in 2001 at the Youth America Grand Prix Finals in New York City. In 2002, she began training at the Harid Conservatory in Boca Raton, Florida, on a full scholarship.
Boylston joined the ABT Studio Company in 2005, the main Company as an apprentice in May 2006 and the corps de ballet in March 2007. She was promoted to Soloist in June 2011 and to Principal Dancer in August 2014.
Her repertory with the Company includes Gamzatti in La Bayadère, the Ballerina in The Bright Stream, Fairy Godmother and the Fairy Summer in Frederick Ashton's Cinderella, Moss in James Kudelka's Cinderella, Aurora in Coppélia, Gulnare and an Odalisque in Le Corsaire, Chloe in Daphnis and Chloe, Kitri and a flower girl in Don Quixote, the second girl in Fancy Free, Lise in La Fille mal gardée, the title role in Alexei Ratmansky's Firebird, Giselle, the peasant pas de deux and Moyna in Giselle, Lescaut's Mistress in Manon, Clara the Princess in The Nutcracker, Olga in Onegin, Other Dances, a Juliet and a Harlot in Romeo and Juliet, Princess Aurora and Princess Florine in Ratmansky’s The Sleeping Beauty, Princess Florine and the Fairy of Fervor in The Sleeping Beauty, Odette/Odile, the pas de trois and the Polish Princess in Swan Lake, the Mazurka in Les Sylphides, Sylvia and Persephone in Sylvia, the Tchaikovsky Pas de Deux, the lead in Theme and Variations, Princess Tea Flower in Whipped Cream and roles in After You, Bach Partita, Ballo della Regina, Birthday Offering, Brahms-Haydn Variations, Brief Fling, Désir, Drink to Me Only With Thine Eyes, Everything Doesn't Happen at Once, In the Upper Room, Her Notes, From Here On Out, Gong, The Leaves Are Fading, Monotones I, Sinfonietta, Songs of Bukovina, Symphony in C and With a Chance of Rain.
She created the Diamond Fairy in Alexei Ratmansky’s The Sleeping Beauty and leading roles in Lauri Stallings' Citizen, Alexei Ratmansky's Chamber Symphony and Dumbarton, Demis Volpi’s Private Light and Christopher Wheeldon's Thirteen Diversions.
She has appeared as a guest artist with the Mariinsky Ballet in St. Petersburg and the Royal Danish Ballet.
Boylston won the 2009 Princess Grace Award and was nominated for the 2010 Prix Benois de la Danse.
In 2011, she received the Clive Barnes Award.
She was the recipient of the 2014 Annenberg Fellowship.